1. What would be an optimum wavelength for measuring the absorbance versus concentration of a series of FD&C Blue 1 dye solutions? Explain your answer. Absorbance measurements are most accurate and sensitive in the range 0.2 – 1.0.
2.Using the information provided in Question 1; predict the absorbance of each solution A-H at the optimum wavelength. Refer to Equation 3 in the Background section. The value of ε is 130,000 M-1 cm-1 .